Abstract
An isocratic high performance liquid chromatographic method has been developed and validated for the estimation of benzoyl peroxide in flours.The benzoyl peroxide was extracted with acetonitrile and analysed by Waters HPLC instrument on a C18 column using water-acetonitrile mobile phase with a flow rate of 1.5 ml/min and UV detection at 235 nm.The linearity was found in the range of 5-50 mg/l.The method is simple,reliable, specific,accurate and precise. KEY WORDS: Benzoyl peroxide, Acetonitrile, HPLC, Method Development,Method Validation,.
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