Abstract
Two monoclonal antibodies (AMb) that react only with Mycoplasma gallisepticum (MG) and do not cross-react with Mycoplasma synoviae (MS) were established. MAb 76 was IgG1 and reacted with a 66 kDa MG protein. MAb 69 was IgG2b and reacted with a 100 kDa MG protein. Using these MAbs as positive controls in an immunoblotting test, 9 of 12 sera from MG inoculated chickens gave a strong band at 66 kDa, and no bands were found at 66 kDa using either 11 sera from MS inoculated chickens or 10 sera from uninoculated, negative control chickens. These results indicated that the 66 kDa protein is a major species-specific protein for MG and that the MAb 76 is a major species-specific monoclonal antibody for MG. the MAb 76 identified 7 of 7 M. gallisepticum strains by a cell-dot-blot method. A coagglutination test using MAb 76 bound to protein A positive Staphylococcus aureus cells was also shown to be a rapid method to identify the seven strains of M. gallisepticum.
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