Development and testing of an endometrial status assessment test based on RNAampliSeq technology

Abstract

BACKGROUND: Approximately two thirds of all implantation failures are due to impaired endometrial receptivity. The lack of implantation may be due to an implantation window failure found in approximately 25-30% of women with a history of failure to perform assisted reproductive technology protocols. The receptivity phase in such patients can be shifted in terms of timing, have a short duration, or not form. Currently, there are several commercial tests for determining endometrial receptivity based on transcriptomic data. However, these tests differ very significantly from each other in the set of genes studied, and the exact mechanism of endometrial receptivity is still not fully understood.
 AIM: The aim of this study was to create and check our own test based on RNA AmpliSeq technology to assess the receptivity status of the endometrium and the implantation window.
 MATERIALS AND METHODS: We previously created an RNAampliSeq panel containing 421 gens. With its use, the differential expression of these genes was analyzed in 38 endometrial samples taken in the proliferative and receptive phases.
 RESULTS: The studied samples form clearly distinguishable clusters with the receptive and proliferative endometria. 271 genes from our panel are differentially expressed in different phases of the menstrual cycle.
 CONCLUSIONS: We have created and tested a model that allows clearly distinguishing between the proliferative endometrium and the receptive one and identifying patients with disorders of the menstrual cycle.