Abstract
We have purified, concentrated and stabilized the protein cytotoxin from Moraxella bovis. The toxin is concentrated on a filter with a 500,000 kDa molecular weight cut off. Small proteins are removed by extensive diafiltration on the same filter. The cytotoxin is concentrated approximately 500 fold, and remains active after 5 months of freezing. A vaccine trial for toxicity and efficacy were performed.
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