Abstract
To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection conditions, DNA vector selection, media change, and incubation time. Adherent or suspension adapted 293-derived cells can be used as production hosts. These results illustrate the importance of optimizing transfection processes for high titer recombinant lentivirus production.
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