Abstract
The purpose of this article is to analyze and compare the carbon footprint value between «in vivo» and «in vitro» microbiological experiments in chicken GIT. The SHIME-style bioreactor for modeling the processes occurring in the gastrointestinal tract of chickens is developed. For «in vitro» estimation – use analytic and experimental results, carrying out on developed chicken GIT model. For « in vivo» estimation, use the carbon footprint of two mounts old broiler chicken. Assessments showed that «in vitro» carbon footprint constitutes about 15% of «in vivo» one. The most significant contributors to «in vitro» carbon footprint are nutrient medium (80%), then control computer (10%), then heather (5%). Recommendations for further carbon footprint reduction for «in vitro» experiments are formulated.
Highlights
At present, the shift of focus from experiments «in vivo» to «in vitro» is an established global trend in microbiology
The processes occurring in the gastrointestinal tract are currently being studied
The studies were carried out on a chicken gastrointestinal tract model developed by a team of researchers as part of a project «Veterinary probiotic drugs for directional modulation of animal health»
Summary
The shift of focus from experiments «in vivo» to «in vitro» is an established global trend in microbiology. Microbiological, and other advantages, conducting a significant part of in vitro research allows reducing the carbon footprint formed during the development of micro- and prebiotic drugs by reducing the contribution from living organisms used in the process of experiments. This requirement is formulated in the 13th UN Sustainable Development Goal in terms of reducing greenhouse gas emissions. Modern technologies allow us to create automated systems that simulate the process of assimilation of food at all its stages These systems include SHIME and TIM [1,2,3].
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