Abstract
Soybean is one of the leading oilseed crop in the world and is showing a remarkable surge in its utilization in formulating animal feeds and supplements. Its dietary consumption, however, is incongruent with its existing industrial demand due to the presence of anti-nutritional factors in sufficiently large amounts. Phytic acid in particular raises concern as it causes a concomitant loss of indigestible complexed minerals and charged proteins in the waste and results in reduced mineral bioavailability in both livestock and humans. Reducing the seed phytate level thus seems indispensable to overcome the nutritional menace associated with soy grain consumption. In order to conceive our objective we designed and expressed a inositol polyphosphate 6-/3-/5-kinase gene-specific RNAi construct in the seeds of Pusa-16 soybean cultivar. We subsequently conducted a genotypic, phenotypic and biochemical analysis of the developed putative transgenic populations and found very low phytic acid levels, moderate accumulation of inorganic phosphate and elevated mineral content in some lines. These low phytic acid lines did not show any reduction in seedling emergence and displayed an overall good agronomic performance.
Highlights
For the last 50 years, world population multiplied more rapidly than ever before, and is expected to grow over a third by 2050
All the transgenic lines analyzed showed a distinct pattern of seperation which indicate that each plant originated from an independent transformation event
The blot showed that all the fragments were above 3 kb in size which suggest that the transgenic lines carry intact copies of T-DNA because the shortest fragments in each line were longer than the T-DNA region (∼2.8 kb)
Summary
For the last 50 years, world population multiplied more rapidly than ever before, and is expected to grow over a third by 2050. One of the major challenges agriculture will face in the coming decades is to meet the food demand of the growing population. Soybean crop due to its unique nutrient profile provide as a promising option to ensure food security in future. United States Department of Agriculture (USDA) estimated that the global soybean production 2017–2018 will be 348.04 million metric tons. Despite such huge production, the presence of natural anti-nutrients, such as inositol hexakisphosphate (Phytic acid, PA), protease inhibitors, lectins, saponins amongst few others, has limited its consumption (Jiang et al, 2013). PA summon attention as it accounts for over 75% of the total seed
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