Abstract
Summary We developed 3 monoclonal antibodies against the common antigen rotavirus which recognize two non-overlapping antigenic sites. These antibodies were selected for their high-affinity to establish an assay with a sensitivity towards the antigen comparable to that of conventional assays with polyclonal antisera. With these particular antibodies, it was possible to carry out an assay with a single 30-min incubation step, achieving a degree of sensitivity almost the same as the Enzygnost-Rotavirus when these methods were tested on our reference strains, 357 unselected stools specimens from children and 41 from neonates. Such a monoclonal ELISA in a one-step procedure gives the advantage of both speed and simplicity and would be applicable to large-scale screening.
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