Abstract
We developed a lateral flow strip using monoclonal antibodies (MAbs) which allows for rapid antigen detection and serotyping of foot-and-mouth disease virus (FMDV). This FMDV serotyping strip was able to detect all 7 serotypes and distinguish serotypes O, A, C and Asia1. Its sensitivities ranged from 103 to 104 of a 50% tissue culture infectious dose of each FMDV stain; this is equal to those of the commercial product Svanodip (Boehringer Ingelheim Svanova, Uppsala, Sweden), which can detect all seven serotypes of FMDV, but does not distinguish them. Our evaluation of the FMDV serotyping strip using a total of 118 clinical samples (vesicular fluids, vesicular epithelial emulsions and oral and/or nasal swabs) showed highly sensitive antigen detection and accuracy in serotyping in accordance with ELISA or RT-PCR. To the best of our knowledge, this is the first report on any FMDV serotyping strip that provides both rapid antigen detection and serotyping of FMDV at the same time on one strip without extra devices. This method will be useful in both FMD-free countries and FMD-infected countries, especially where laboratory diagnosis cannot be carried out.
Highlights
Foot-and-mouth disease (FMD) is one of the most highly contagious viral diseases, and causes devastating economic damage in the countries affected by it
We developed a lateral flow assay using monoclonal antibodies (MAbs) (FMDV serotyping strip), which allows for rapid FMD antigen detection for all 7 serotypes and FMD serotyping for types O, A, C and Asia1, in the field, and especially in countries where laboratory diagnosis cannot be carried out, in vast countries in which it would take a long time to transport clinical samples to a laboratory, and/or in countries lacking transportation facilities
We developed an FMD serotyping strip that enables rapid antigen detection for all 7 serotypes and serotyping for O, A, C and Asia1, including recent epidemic foot-andmouth disease virus (FMDV)
Summary
Foot-and-mouth disease (FMD) is one of the most highly contagious viral diseases, and causes devastating economic damage in the countries affected by it. FMD is caused by foot-andmouth disease virus (FMDV), which belongs to the Aphthovirus genus of the family Picornaviridae, and consists of 7 immunologically distinct serotypes: O, A, C, Asia, and South African Territories (SAT) 1, 2 and 3. There may be many different topotypes [1]; serotype A in particular displays a large number of topotypes [2]. In Asia, type O, A and Asia viruses have prevalently existed and the disease is endemic or sporadic in main countries [3]. There have been some cases of FMD whose serotype is unknown in South.
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