Development and Evaluation of a Multiplex PCR for Simultaneous Detection of Five Foodborne Pathogens

Giau Vo Van,Thuy Trang Nguyen,Tuong Kha Vo

doi:10.15226/2475-4714/1/3/00114

Abstract

Foodborne pathogens present serious concerns to human health and can even lead to fatalities. The gold standard for pathogen identification – bacterial culture – is costly and time consuming. A cheaper and quicker alternative will benefit in controlling food safety.

Highlights

The incidence of foodborne diseases has increased over the years and is a serious health hazard in both developing and developed countries
Rapid and simultaneous detection of multiple pathogenic bacteria in foods is of great importance to ensure food safety
We developed and evaluated a multiplex polymerase chain reaction (PCR) for simultaneous detection of the five food borne pathogens including E. coli O157:H7, S. aureus, Salmonella spp, L. monocytogenes and V. cholera in a single reaction

Summary

Introduction

The incidence of foodborne diseases has increased over the years and is a serious health hazard in both developing and developed countries. Escherichia coli O157:H7 (E. coli O157:H7), Salmonella spp., and Vibrio cholera (V. cholera) are likely the most common cause of foodborne disease [1, 2]. The well-known E. coli bacteria that produce Shiga toxin (STEC) is E. coli O157:H7 strains are foodborne infectious agents that cause a number of life-threatening diseases, including hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) [3]. Shiga toxin (Stx) is one of the major virulence factors involved in E. coli O157:H7 pathogenesis [5]. 20,000 hospitalizations and 378 deaths per year in the United States, Salmonella spp. are the leading bacterial cause of acute gastrointestinal illness. The invA gene is a good candidate gene to invade mammalian cells and subsequently cause disease [14,15], and it presents in all pathogenic serovars as a maker has been the most frequently used for Salmonella spp.

Methods

Results

Conclusion