Abstract
Objective: The lack of possible cure and associated disadvantages of allopathic medicines in the topical treatment of psoriasis has led to extensive research for anti-psoriatic activities of plant-based drugs. The present study was aimed to develop topical cream formulation containing the Nanostructured Lipid Carriers of Azadirachta indica leaves extract (AE), Lawsonia inermis leaves extract (LE) and fruit extract of Mallotus philippensis (ME) and assessing the antipsoriatic activity of prepared cream formulation.
 Methods: The Drug loaded NLCs were prepared via hot homogenization technique and incorporated into the water in oil (w/o) cream base that was prepared by the emulsification method. Evaluation of cream formulation included advanced preclinical trials using Rat Ultraviolet Ray-B photodermatitis and Mousetail method, anti-lipid peroxidation assay, nitric oxide scavenging activity, cytotoxicity and DNA fragmentation on HaCat cell lines to ascertain antipsoriatic potential and in vitro drug diffusion.
 Results: In spite of the low amount of the drug loading in NLCs their potency was much higher as displayed in Advanced Preclinical studies and cell line studies for psoriasis indicating the suitability of NLCs loaded creams for skin applications due to their various desirable effects on the skin. Occlusive properties of the prepared NLC on the skin provide an increase in drug penetration particularly via skin moisturization. The in vitro drug diffusion studies suggest the prolonged and almost complete release of AE, LE and ME from NLC based cream up to 24 h that was found to be 81.24±0.51%, 74.31±0.25% and 70.43±0.78% respectively. The literature survey supports the novelty of such topical anti-psoriatic polyherbal nanoparticulate cream.
 Conclusion: It can be concluded that the AE, LE and ME loaded NLC based cream can be used for prolonged topical delivery of drugs for the management of Psoriasis.
Highlights
Psoriasis is an autoimmune skin disease that causes scaling and inflammation of the skin and occurs when the immune system sends out faulty signals that speed up the growth cycle of skin cells than normal [1,2]
The literature survey reveals that a maximum of 5-10% of the drug can be loaded in NLCs [12]
Our preliminary studies have shown the encouraging results for antipsoriatic potential of the dried extracts of Azadirachta indica, Lawsonia inermis, and Mallotus philippensis when loaded in Nanostructured Lipid Carriers [13, 14]
Summary
Psoriasis is an autoimmune skin disease that causes scaling and inflammation of the skin and occurs when the immune system sends out faulty signals that speed up the growth cycle of skin cells than normal [1,2]. The literature survey reveals the multitude of medicinal properties of Azadirachta indica, Lawsonia inermis, and Mallotus philippensis for the treatment of skin diseases as well as absence of such combination therapy [6]. Our preliminary studies have shown the encouraging results for antipsoriatic potential of the dried extracts of Azadirachta indica, Lawsonia inermis, and Mallotus philippensis when loaded in Nanostructured Lipid Carriers [13, 14]. The present study was aimed to develope topical cream formulation containing the Nanostructured Lipid Carriers of Azadirachta indica leaves extract (AE), Lawsonia inermis leaves extract (LE) and fruit extract of Mallotus philippensis (ME) and assessing the antipsoriatic activity of prepared cream formulation using cell line studies and preclinical trials on animal models
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