Abstract
Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species.
Highlights
The Sedum L. genus in the Crassulaceae family consists of 470 species that are distributed in the Northern Hemisphere, Africa and Latin America of Southern Hemisphere [1]
Sedum L. species are widely used in folk medicine, especially Sedum sarmentosum, which has been frequently used for the treatment of chronic hepatitis in China [2,3]
Fragments containing microsatellite motifs that showed flanking regions were used to design primers and tested for consistency of amplification and polymorphism with 48 S. sarmentosum
Summary
The Sedum L. genus in the Crassulaceae family consists of 470 species that are distributed in the Northern Hemisphere, Africa and Latin America of Southern Hemisphere [1]. The species of S. sarmentosum are difficult to identify because of the large variety and close resemblance in closely related genera. It is usually morphologically confused with S. lineare [6]. DNA markers, such as random amplified polymorphic DNA, have been established to study the genetic relationship of six Sedum species. The first set of genomic polymorphic microsatellite markers of S. sarmentosum have been developed using a microsatellite-enriched library and tested for transferability across five other Sedum species. The polymorphic microsatellite markers might have important implications for the identification, genetic diversity and population genetic structure of Sedum genus
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