Abstract
Abstract Monolithic poly(octadecyl methacrylate- co -ethylene dimethacrylate) capillary columns for use in capillary electrochromatography (CEC) were developed and characterized by porosimetric measurements and scanning electron microscopy (SEM). The stationary phases were prepared using 2-acryloylamido-2-methylpropanesulfonic acid (AMPS) as the ionizable monomer and 2,2′-azobisisobutyronitrile (AIBN) as the initiator of thermal polymerization, which occurred at 60 °C. The porogenic solvents used in this work were amyl alcohol and 1,4-butanediol, in the proportion 65:35 (v/v). The ratio between monomers and porogenic solvents was varied in a range of 60 to 80% (v/v) of porogenic agents. The porosimetry showed that the increase in content of porogenic solvents caused an increase in pore surface area, but a relationship with the separation efficiencies was not observed. SEM agreed with the conclusions made through porosimetry, where decreases in globule size could be noted with increases in porogenic solvents.
Published Version
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