Abstract
The dojo loach Misgurnus anguillicaudatus is an endemic freshwater species in Asia. The effective conservation and molecular-aided selection of M. anguillicaudatus were limited without sufficient molecular markers. In this study, 112 novel single nucleotide polymorphism (SNP) markers were screened based on 2b-RAD sequencing database, and 56 SNPs of them were developed and characterized by genotyping 40 individuals using SNaPshot method. The observed heterozygosity (Ho) ranged from 0.025 to 0.675, while the expected heterozygosity (He) varied from 0.025 to 0.500. The minor allele frequency (MAF) ranged from 0.013 to 0.500. Among these SNPs, 18 loci were found to deviate significantly from the Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.05). The first set of SNP markers developed from M. anguillicaudatus will provide valuable information in further population genetic analysis and natural resource conservation.
Highlights
The dojo loach Misgurnus anguillicaudatus, a member of the family Cobitidae, is an endemic freshwater species in Asia and is widely distributed along the eastern coasts of the Asian continent (Li et al 2008; Zeng et al 2012)
The effective conservation and molecular-aided selection of M. anguillicaudatus have been limited without sufficient molecular markers
The first set of single nucleotide polymorphisms (SNPs) markers developed from M. anguillicaudatus will provide valuable information in further population genetic analysis and natural resource conservation
Summary
The dojo loach Misgurnus anguillicaudatus, a member of the family Cobitidae, is an endemic freshwater species in Asia and is widely distributed along the eastern coasts of the Asian continent (Li et al 2008; Zeng et al 2012). The research of genetic markers in M. anguillicaudatus has mainly concentrated on mitochondrial DNA and microsatellite marker at present (Zhang et al 2013; Fujimoto et al 2017; Lee et al 2018) Owing to their wide genomic distribution and high level of polymorphism, single nucleotide polymorphisms (SNPs) have gradually become one of the most important DNA markers for genetic studies (Vignal et al 2002; Seeb et al 2011). The PIC ranged from 0.024 to 0.375 (Table1) Among these SNPs, 18 loci showed significant deviations from the Hardy–Weinberg equilibrium after Bonferroni correction (P < 0.05). To our knowledge, this is the first development of SNP markers in M. anguillicaudatus, and these novel markers offer a valuable tool for future studies of population conservation and natural resource conservation in this species
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
