Development and application of a new QuEChERS-molecularly imprinted solid phase extraction (QuEChERS-MISPE) technique for analysis of DDT and its derivatives in vegetables

Abstract

The current study synthesized a molecularly imprinted polymer, combined it with the QuEChERS method to form a new hybrid technique, the QuEChERS-MISPE as an alternative to the QuEChERS-dSPE for analysis of DDTs as model pesticides. Batch studies confirmed that the binding of the DDTs to the MIP cavities formed a monolayer formation through chemisorption resulting in an adsorption capacity of 429 ng g−1. A study of matrix effects indicated signal suppression for both techniques. However, the new QuEChERS-MISPE technique is less affected by matrix effects, has better sensitivity and recoveries compared to the conventional QuEChERS-dSPE technique. Application of the new QuEChERS-MISPE technique detected trace levels of DDTs in vegetables in South Africa. However, a health risk assessment indicated that potential risks for consumers was minimal. Although the risk is minimal, the detection of DDTs in vegetables in South Africa should be a concern and more constant monitoring is required.