Developing regulatory property of gelatin-tannic acid multilayer films for coating-based nitric oxide gas delivery system

Kyungtae Park,Junjira Tanum,Hyejoong Jeong,Jae-Chan Yoo,Jinkee Hong

doi:10.1038/s41598-019-44678-2

Kyungtae Park, Junjira Tanum + Show 3 more

Open Access

https://doi.org/10.1038/s41598-019-44678-2

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Abstract

To utilize potentials of nitric oxide (NO) gas in anti-bacterial, anticancer, wound healing applications, numerous studies have been conducted to develop a NO delivery system in the past few decades. Even though a coating method and film types are essential to apply in biomedical device coating from previous NO delivery systems, release control from the coating system is still challenging. In this study, we introduced a multilayered polymeric coating system to overcome the uncontrollable NO release kinetics of film systems. We used biocompatible gelatin and tannic acid to construct a rough, porous structured film based on the layer-by-layer self-assembly method. The multilayered polymeric structure facilitated the controlled amount of NO release from (Gel/TA)n film and showed burst release in early period owing to their large surface area from the rough, porous structure. We synthesized the proton-responsive NO donor, N-diazeniumdiolate (NONOates), into the (Gel/TA)n film through a chemical reaction under high pressure NO gas. NO release profile was analyzed by a real-time NO analysis machine (NOA 280i). Then, the NO-releasing (Gel/TA)n film was tested its toxicity against human dermal fibroblast cells and bactericidal effects against Staphylococcus aureus.

Highlights

Nitric oxide (NO) is an endogenously produced gas molecule from L-arginine by three nitric oxide synthase (NOS) enzymes in the human body[1,2]
The porous structure might be formed by a hydrophobic pocket between the hydrophobic side chain of gelatin and aromatic rings of tannic acid (TA), which was stabilized by hydrogen bonding[28]
From the film growth data and the surface morphology, we determined that the (Gel/TA)n film could be successfully constructed at the certain pH condition and porous structure attributed to the exponential increase

Summary

Introduction

Nitric oxide (NO) is an endogenously produced gas molecule from L-arginine by three nitric oxide synthase (NOS) enzymes in the human body[1,2]. We used natural materials Gel and TA that emphasized their biocompatible properties in the previous paragraph to develop a polymer-based coating system to improve the controlled release ability of NO delivery by constructing gelatin and tannic acid multilayer (Gel/TA)n.(here, n means number of bilayers). We fabricated the multilayer film using the LbL self-assembly technique with different numbers of bilayers, analyzed the film characteristics measuring the thickness growth tendency and the surface morphology.

Results

Conclusion