Abstract
Technological developments over the past century have made microbes the work-horses of large scale industrial production processes. Current efforts focus on the metabolic engineering of microbial strains to produce high levels of desirable end-products. The arsenal of the contemporary metabolic engineer contains tools that allow either targeted rational interventions or global screens that combine classical approaches with –omics technologies. Production of terpenoids in S. cerevisiae presents a characteristic example of contemporary biotechnology that integrates all the variety of novel approaches used in metabolic engineering. Terpenoids have attracted significant interest as pharmaceuticals, flavour and fragrance additives, and, more recently, biofuels. The ongoing metabolic engineering efforts, combined with the continuously increasing number of terpene biosynthetic enzymes discovered will enable the economical and environmentally friendly production of a wide range of compounds.
Highlights
Microbial fermentation processes have been global screens that combine classical approaches of strain evolution extensively used for the processing of foods and the production of through adaptation and selection with –omics approaches that can beverages, while technological developments over the past century globally assess changes leading to desirable outputs
Thessaloniki, Greece set of targeted interventions to increase the pool of the intermediates GPP, FPP, and GGPP, which are the substrates of terpene synthases
Targeted interventions in the yeast MVA pathway to increase the terpene substrate pool stabilization of the endogenous Hmg proteins and to dramatic improvements in sesquiterpene production that could not be achieved by tHmg2p overproduction alone (Ignea, submitted)
Summary
Microbial fermentation processes have been global screens that combine classical approaches of strain evolution extensively used for the processing of foods and the production of through adaptation and selection with –omics approaches that can beverages, while technological developments over the past century globally assess changes leading to desirable outputs. Manipulation of microbial metabolism holds major advantages, since chemical diversity of natural product structures [2]. Members of this microbes offer an environmentally friendly means to efficiently group have attracted commercial interest as flavour and fragrance convert cheap raw materials like glucose, sucrose, and biomass derived additives in the food and cosmetic industry. There is continuous development and improvement of yeast taxol, a diterpene from yew, which has successfully been established as strains for the production of high levels of desirable end products. The pathway has been extensively studied for several years, since it functioned as a model for understanding human aSchool of Medicine, University of Crete, P.O. Box 2208, Heraklion 71003, disease caused by high cholesterol levels [16] and is target of an Greece important class of antifungal compounds [16]. Thessaloniki, Greece set of targeted interventions to increase the pool of the intermediates GPP, FPP, and GGPP, which are the substrates of terpene synthases
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