Abstract
Terpenoids are a large family of natural products with remarkable diverse biological functions, and have a wide range of applications as pharmaceuticals, flavors, pigments, and biofuels. Synthetic biology is presenting possibilities for sustainable and efficient production of high value-added terpenoids in engineered microbial cell factories, using Escherichia coli and Saccharomyces cerevisiae which are identified as well-known industrial workhorses. They also provide a promising alternative to produce non-native terpenes on account of available genetic tools in metabolic engineering and genome editing. In this review, we summarize the recent development in terpenoids production by synthetic biology approaches.
Highlights
Terpenoids, known as terpenes or isoprenoids, are a large family of natural products
Two-phase system; convert monoterpenoid geraniol to its acetyl ester to avoid geraniol toxicity Promoters and RBSs engineering Clustered regularly interspaced short palindromic repeats interference (CRISPRi)-guided balancing of MVA pathway FPP-resistant mevalonate kinase 1; lower MVA pathway; Optimization of inducer concentration, aeration and enzymatic cofactor Modular engineering (MEP, cyclase, and P450 module), promoters engineering Codon optimization of longifolene synthase, investigate into different FPP synthases Ophiobolin synthase with small ubiquitin-like modifier (SUMO) tag; phylogenetics based mutation Scaffold-free enzyme assemblies (IDI and CrtE) Promoters and RBSs engineering; multidimensional heuristic process (MHP) CRISPR-mediated morphology and oxidative stress engineering Dynamic control of MVA pathway by isopentenyl diphosphate (IPP)/FPP-responsive promoter
The enormous potential of E. coli and S. cerevisiae as platform strains has been confirmed with various successes
Summary
Terpenoids, known as terpenes or isoprenoids, are a large family of natural products. Using IPP/FPP-responsive promoter in E. coli, Shen et al (2016) coordinated the expression of all genes of the MVA pathway from S. cerevisiae using the tunable intergenic regions to increase the availability of FPP.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
