Abstract
The recent emergence of DNA-based diagnostics increases the need for rapid DNA sequencing technologies. One method to achieve this is to pass DNA through a nanopore, recording the trans-membrane current with a low-noise current amplifier. The challenge presented in this method is that the bandwidth of commercially available current amplifiers is limited to 100kHz, which is not fast enough to resolve the signals present in DNA sequencing. To overcome this problem, researchers have developed a custom amplifier that has reached a bandwidth of approximately 1MHz, being able to reveal additional information during the DNA translocation events through a nanopore.We will demonstrate a design of a custom amplifier that offers a wider bandwidth than the current designs, enabling the study of DNA translocation without the need to limit the speed of translocation. In addition, an amplifier with a bandwidth larger than 1MHz allows discoveries to be made about information that might be present in a higher range of frequencies, enabling measurements at a higher time resolution than what was previously possible. The amplifier will be designed to allow direct integration of a micro- or nanopore sensing area on the same physical substrate, eliminating the need for external electrode wiring. The outcomes from this research open up the possibility of an integrated high-speed DNA sequencer chip enabling rapid disease diagnostics.
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