Abstract
In this study, the exopolysaccharide from cyanobacteria was used for detoxification of acid hydrolysate of water hyacinth biomass. Exopolysaccharide-hydrogel showed phenolics and furans removal of 86 % and 97 %, respectively, with sugar recovery of 98.3 %. The fermentation of detoxified acid hydrolysate was integrated with that of pretreated biomass subjected to enzymatic saccharification derived from commercial cellulose (ESF) or from microbe (MSF). The maximum hydrogen production of 69.2 mL/g-VS was obtained in MSF, which is 1.2- and 1.6-fold higher than ESF and undetoxified acid hydrolysate, respectively. Additionally, the methane production of 12.6 mL/g-VS by mixed methanogenic consortia was obtained using the spent liquor containing volatile fatty acids. This enhanced hydrogen and methane production in subsequent microbial processes is mainly attributed to the selective removal of inhibitors in combination with an integrated carbohydrate utilization.
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