DETOXIFICATION OF USED SYNTHETIC MOTOR OILS WITH BIOCOMPOSITIONS BASED ON HUMIC ACIDS IN WATER

Abstract

Among the modern technologies of remediation of oil-contaminated wastewater, the most promising are technologies based on biological methods of purification, namely, on the process of self-purification using various consortia of microorganisms characterized by an increased ability to biodegrade hydrocarbons of oil and petroleum products. This article is devoted to the assessment of the detoxifying ability of new biocompositions based on microorganisms-oil destructors in combination with natural dispersants – humic acids in relation to used synthetic motor oil in water. Biocompositions based on humic acids isolated from the peat of the Tula region: reed lowland and black-alder lowland, and microorganisms-oil destructors of the genus Rhodococcus, producing biological surfactants and capable oxidizing a wide range of petroleum hydrocarbons due to the presence of enzymatic systems, have been studied. The detoxifying ability of biocompositions was determined by the method of biotesting by assessing the toxic effect on the test object duckweed small. The reduction of the toxic effect by the detoxification coefficient was achieved by more than 50%. Attention is focused on the advantages of biotesting in comparison with analytical methods, which consist in ease of use, expressiveness in determining results, cost–effectiveness and, most importantly, the ability to judge how dangerous a specific amount of xenobiotic is for the vital activity of organisms. The experiments carried out prove that the developed biocomposition affects the dispersed state of used synthetic engine oil in water, and also contributes to the degradation of its components. The obtained biocompositions can be used as complex biological products for biotechnological remediation of aquatic environments using in situ technology.