Determinism of the induction of the reproductive cycle in female Eurasian perch, Perca fluviatilis: Identification of environmental cues and permissive factors

Abstract

In Eurasian perch, Perca fluviatilis, gametogenesis is induced by decrease of both temperature and photoperiod. However, a multiplicity of other factors can display non-permissive or modulating effects on the induction of the reproductive cycle. Consequently, a 9-week study was carried out to determine the environmental cues and permissive factors controlling the induction of the reproductive cycle in this species. A two-level fractional factorial design 2 8–4 was used in order to identify the influent factors from 8 environmental and nutritional ones and their 28 interactions. Tested factors were: (1) amplitude of temperature decrease, (2) time, (3) kinetics and (4) amplitude of photoperiod decrease, (5) initial nutritional state, (6) handling, (7) feeding rate and (8) light spectrum. Increase of gonadosomatic index (GSIi), proportion of female in exogenous vitellogenesis (EVP), plasma 17 β-estradiol (E 2) and cortisol levels, fat consumption and food intake were evaluated. This study showed that the amplitude of the decrease of temperature and the time of photoperiod decrease are the two main environmental cues controlling the induction of the reproductive cycle in Eurasian perch. GSIi was the highest (3.8%) when females were exposed to both low amplitude of temperature decrease and precocious decrease of photophase. It was the lowest (1.1%) when high amplitude of temperature decrease and late decrease of photophase were applied. Handling in association with temperature modulated the broodstock response. 100% of the unhandled fish held at 14 °C were at the exogenous vitellogenesis stage with mean E 2 and basal cortisol levels of 1.6 ng mL − 1 and 9 ng mL − 1 respectively. Handled fish and fish held at 6 °C exhibited lower vitellogenesis (40–73%) associated with lower E 2 (0.6–1.1 ng mL − 1 ) and higher basal cortisol levels (37–89 ng mL − 1 ). No other factor nor interaction displayed a cueing or non permissive effect on the induction of the reproductive cycle in female Eurasian perch. A first schematic model is proposed to explain the factorial determinism of the induction of the reproductive cycle.