Determining whether positively-charged channel-forming molecules of polyene antibiotic with aromatic groups affect muscle activity?

Abstract

This article evaluates the effect of membrane active channel-forming polyene antibiotic (PA) of levorin and its alkyl derivatives on the muscle performance. The membrane channels of muscle cells are capable to transport ions of potassium, sodium, and calcium. In the period of an intensive muscle exercise, the necessity for organic substrates increases and these channels start to work with the greater intensity. However, specific activity of native channels is limited, and, therefore they have no capacity to deliver organic compounds to muscle cells in indispensable quantity. The power engineering of muscle performance weakens rapidly and then, the muscle becomes fatigued. Here there is a necessity for activation of native cell channels induced by exogenic drugs. For this purpose, channel-forming substances like polyene antibiotics have been studied. To test the quality of such a system, BLM was used. In this technique, amphotericin B and levorin is incorporated in a lipid membrane to simulate the process of ionic permeability for such ions as potassium, sodium, calcium, and also transmembrane transport of carbohydrates and other low-molecular weight compounds. The results showed that amphotericin B and levorin enhance lipid membrane permeability for ions and monosaccharides and other neutral moleculas in the following order: water>urea>acetamide>glycerine>ribose>arabinose>glucose>saccharose. Thus, the use of PA and their derivatives with established chemical structure will create an indispensable condition for strengthening synthesis of energy-dependent substrates in muscle cells and may increase their membrane potential and can maintain energy potential of the organism at a high level. It was also demonstrated that aromatic heptayene antibiotic levorin increases the permeability of membranes for monosucrose and other neutral molecules in the following order: H2O>urea> acetamide>glycerine>ribose>arabinose>glucose>saccharose.