Abstract

Knowledge of the structure of fluid lipid bilayers is essential for understanding complex biological phenomena in cellular membranes. Water, in particular, is an important component of the membrane since membrane proteins anchor and function in cellular membranes through interactions with water and lipid polar headgroups. Here we present an experimental method to determine directly the content of water in a membrane, at thermodynamic equilibrium with its environment, which does not require knowledge of the density of water.

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