Abstract
Hydrogen fermentation is a very complex process and is greatly influenced by many factors. Previous studies have demonstrated that temperature, pH and substrate are important factors controlling biological H 2 production. Response surface methodology with central composite design was used in this study to optimize H 2 production from glucose by an anaerobic culture. The individual and interactive effects of pH, temperature and glucose concentration on H 2 production were also evaluated. The optimum conditions for maximum H 2 yield of 1.75 mol-H 2 mol-glucose −1 were found as temperature 38.8 °C, pH 5.7 and glucose concentration 9.7 g L −1. The linear effects of temperature and pH as well as their quadratic effects on H 2 yield were significant, while the interactive effects of three parameters were minor.
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