DETERMINING M235T POLYMORPHISM OF ANGIOTENSINOGEN GENE (AGT) IN NORMOTENSIVE AND PREECLAMPTIC/ECLAMPTIC PREGNANT WOMEN

Abstract

Background: The 235T allele of AGT gene has been reported to be associated with higher AGT level and abnormal remodeling of the uterine spiral arteries, which is an early cause preeclampsia/eclampsia (PE/E). The aim of this study was to: (1) determine the frequencies of 235MM, 235MT and 235TT genotypes of AGT gene in normotensive and preeclamptic/eclamptic pregnant women, and (2) survey the association between AGT M235T polymorphism and PE/E. Patients and methods: 68 preeclamptic pregnant women and 272 normotensive pregnant women were determined M235T genotypes of AGT gene by allele – specific PCR technique with DNA samples extracted from whole blood. Results: The frequencies of 235MM, 235MT and 235TT genotypes in normotensive pregnant women were 1.8%; 15.8% and 82.4%, respectively, the distribution of these genotypes was in Hardy – Weinberg equilibrium. These frequencies in preeclamptic/eclamptic pregnant women were 1.5%; 14.7% and 83.8%, respectively. The frequencies of 235T allele in two groups were 90.3% and 91.2%, respectively. There was no association between the M235T polymorphism and PE/E in all of models, including 235TT/(235MT + 235MM), (235TT + 235MT)/235MM, 235TT/235MM and 235T allele/235M allele. Conclusion: The frequencies 235T allele in both groups were very high. There was no association between M235T polymorphism and preeclampsia/eclampsia. Keywords: Preeclampsia, M235T polymorphism, AGT gene