Abstract
A new automated method for the quantitative determination of ascorbic acid in the substance, injection forms, and tablets has been developed and validated. The proposed method is based on two versions of capillary electrophoresis: (i) capillary zone electrophoresis and (ii) micellar electrokinetic chromatography. The method shows high accuracy, repeatability, and precision (with both within-run and between-run variation coefficients lower than 1%). The method measures ascorbic acid in a linear manner (r = 0.999) with a detection limit of 8.8 mg/L. The quantity of ascorbic acid in injections amounted to 49.99 ± 0.24 mg/mL; in tablets, 49.39 ± 0.15 mg.
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