Determination on Antioxidant Activity of Sansevieria cylindrica Bojer ex Hook. Leaf Extract

Abstract

Antioxidant compounds work by preventing or detecting oxidative chain reactions caused by free radicals, stabilizing generated radicals and thus reducing the risk of oxidative damage in the body. They are sometimes called as free radical scavengers. Plants are naturally rich in antioxidants which contains bioactive compounds with high antioxidant activity. The work was carried out to verify whether different types of method shows the same sensitivity and/or capacity to determine the antioxidant activity of two different extract of Sansevieria cylindrica Bojer ex Hook. Their extracts were analyzed using 4 four different methods (i) DPPH (ii) Hydrogen peroxide (iii) Superoxide (iv) Nitric Oxide scavenging activities. These four methods could quantify the antioxidant capacity. Antioxidant evaluation methods must be fast, reproducible, require small amounts of the chemical compounds being analyzed, and not be affected by the physical characteristics of the compounds. The highest antioxidant activity was observed in both the extracts 13.6% (Ethanolic extract) and 13.9% (Chloroform extract) in 20µg/ml concentration of DPPH activity and the lowest antioxidant activity was also observed in ethanolic extract of Nitric oxide radical scavenging activity 68.7% in 100µg/ml concentration. Finally the application of DPPH free radicals are higher than the results of other three methods of free radical activity.