Abstract
Zinc is an essential micronutrient for gestation, its determination in different compartments of organism during pregnancy is important to value the zinc homeostasis, as in serum and urine, and the determination by atomic absorption spectrophotometry (AAS) is the method par excellence, but in institutions of low infrastructure does not have this equipment, being therefore an alternative by colorimetric measurement. In this study was adapted a protocol for measuring zinc in serum and urine from 54 pregnant women attending public hospital Baranoa Township, Atlantic -Colombia-, adapted through the colorimetric method using Zincon described by William et al (1962 ) and Methods for the examination of Water and Waste Water , 17th, (2005). Samples were collected from each women in fasting of 12 hours, at first (8-12 weeks, 1T), second ( 20-25 weeks, 2T ) and third (30-36 weeks, 3T) trimester of gestation. Zinc values in serum were: 80.3±12.2; 60.0±9.0; 55.8±14.3 µg/dL, and in urine: 17.8±5.20; 16.0± 6.5; 14.3±9.1 µg/dL, for 1T, 2T and 3T, respectively. Values decreased throughout gestation, 30 % from 1T to 3T for serum and 20% for urine, similar to observed in others studies. Although the colorimetric method is not comparable to that of AAS, seems to be an alternative to measurements zinc in longitudinal studies in low-resource institutions, using the zincon as colorimetric complexing.
Published Version
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