Abstract
Cholesterol ester (ChE) and triacylglycerol (TG) hydroperoxides in human plasma were determined by high-performance liquid chromatography with postcolumn detection with diphenyl-1-pyrenylphosphine. Human plasma was extracted once with n-hexane. 2,6-Di- tert.-butyl-4-methylphenol and N-stearylcinnamide were added to human plasma before extraction as an antioxidation agent and an internal standard, respectively. The detection limits of both ChE and TG hydroperoxides were 1 pmol. The sample size was minimized to 250 μl for each run. The recoveries of ChE and TG hydroperoxides from fresh plasma were ca. 90 and 80%, respectively. The relative standard deviations ( n = 8) of their values in frozen human plasma were 5.4% (ChE hydroperoxides, 298 n M) and 5.7% (TG hydroperoxides, 267 n M). No TG hydroperoxides and 24.5 ± 9.6 n M ( n = 15) ChE hydroperoxides were detected in fresh human plasma. The relative standard deviation ( n = 8) of ChE hydroperoxides values in fresh plasma was 5.8% (27.1 n M).
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