Abstract
A capillary electrophoresis (CE) coupled with end-column electrochemiluminescence (ECL) detection method for the analysis of tramadol (TMD) has been investigated. ECL detection was working electrode biased at 1.2 V in a 20mmol·L-1 sodium phosphate buffer (pH = 8.0) containing 5 mmol·L-1 Ru (where bpy = 2,2’-bipyridyl). Linear correlation (r ≥ 0.997) between ECL intensity and drug concentration was obtained in the range 3 × 10-4 - 6 × 10-6 mol·L-1. The limits of detection (LODs) for tramadol in water was 3.012 × 10-8 mol·L-1(S/N = 3). The relative standard deviation values on peak size (10-5 mol·L-1 level) and migration time for the tramadol were 4.58% and 1.39% (n = 10), respectively. Applicability of the CE-ECL method to the analysis of human serum spiked with tramadol was examined
Highlights
Tramadol (Figure 1) hydrochloride is a new category of non-morphine analgesic drugs, which can alleviate the ache because of bone fracture or each technique, the mild cancer
Capillary electrophoresis (CE) had attracted much attention and became an important and powerful analytical tool in liquid separation and detection areas [3] including the analysis of inorganic ions, organic molecules, clinic and pharmaceutical fields [4]
Multi-channel data acquirement and analysis apparatus was employed to collect the intensity of the electrogenerated chemiluminescence
Summary
Tramadol (Figure 1) hydrochloride is a new category of non-morphine analgesic drugs, which can alleviate the ache because of bone fracture or each technique, the mild cancer. A sensitive method to determine tramadol at low level is of considerable for pharmacokinetic and clinical studies In these methods, the ultraviolet (UV) [1], high-performance liquid chromatography (HPLC) [2], capillary electrophoresis gas chromatography (CEGC), and bolivian carbon electrode voltammetry are commonly used. CE technique has advantages of minimal sample volume, high separation efficiency, relatively short analysis time and low instrumental cost [5]. These advantages make CE an alternative to HPLC. A simple, fast, sensitive and more selective method for detecting tramadol is presented and successfully used to determine the tramadol contents in human serum sample
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