Abstract
The tricyclic antidepressants (TCA) imipramine (Imi) and trimipramine (Tri) were successfully analyzed by capillary electrophoresis (CE) coupling with Tris(2,2-bipyridyl) ruthenium(II)-based (Ru(bpy)32+) end-column electrochemiluminescence (ECL) detection. The addition of β-CD to the running buffer was found to enable baseline separation of the two analytes and the addition of acetonitrile (ACN) as an organic additive to improve the repeatability and sensitivity of the CE method. Under the optimized separation and detection conditions (50mM PBS (pH=7.0) and 2mM Ru(bpy)32+ in the ECL detection cell, 20mM Tris (pH=2.0), 0.2mM β-CD and 20% ACN (v/v) as running buffer), wide linear ranges of 0.1–5μM and 0.1–5μM were achieved, with the correlation coefficients of 0.9990 (n=8) and 0.9980 (n=8) for Imi and Tri, respectively. Detection limits 5nM and 1nM (S/N=3) were obtained for Imi and Tri, respectively. The method was also successfully applied for the determination of Imi in pharmaceutical dosage form.
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