Abstract
A method for the determination of trace levels of transition metal ions such as Fe 2+, Mn 2+ and Zn 2+ by liquid chromatography using reversed-phase ion-pair separation, post-column derivatization with 4-(2-pyridylazo)resorcinol and spectrophotometric detection at 520 and 540 nm is described. Even high levels of typical fermentation media constituents such as sugars and citric acid do not have a negative effect on the determination. The application of the method to μg 1 −1 levels of Fe 2+ and Mn 2+ ions in citric acid fermentation broth suffers from relatively high oscillating noise signals and unpredictable retention times, which is caused by the column capacity being exceeded at high concentrations of cations such as K +, Mg 2+ and Ca 2+ and certain organic substances. Digital filtering procedures were used to improve the signal-to-noise ratio and correct peak identification was ensured by determining the absorption ratio A 540/ A 520.
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