Determination of trace bismuth by atomic fluorescence spectrometry in blood

Abstract

Objective: To establish a method for determination trace bismuth in blood by atomic fluorescence spectrometry (AFS) . Methods: 4.0 ml nitric acid and 1.0 ml perchloric acid was added into 1.0 ml blood sample then through automation graphite digestion instrument digested, after that 1.0 ml thiocarbamide-vitamin (10%) was injected, 8% HCl constant volume to 10.0 ml, the bismuth was detected by atomic fluorescence spectrometry with 5.0 ml digestive sample. Results: The method showed a linear relationship within the range of 0.4-50.0 μg/L (r=0.999 7) . The within-run and between-run relative standard deviations (RSD) of repetitive measurement at 10.0, 20.0, 40.0 μg/L concentration levels were 2.2%-4.9% and 3.0%-4.0%. The detection limit was 0.032 μg/L. The recoveries of bismuth were 93.0%-103.9%. Conclusion: This method is low detection limit, good accurate and high sensitivity. It has been applied for determination of trace bismuth in blood samples those who need occupation health examination or poisoning diagnosis.