Determination of Toxic Elements in Muscle Tissues of Five Fish Species Using Ultrasound‐Assisted Pseudodigestion by Electrothermal Atomic Absorption Spectrophotometry: Optimization Study

Abstract

A simple and rapid method is described for the determination of As, Cd, and Pb in muscle tissue of five freshwater fish species by ultrasound‐assisted pseudodigestion (USD). A Plackett‐Burman experimental design was used as a multivariate strategy for the evaluation of the effects of varying several variables at once. The variables such as sonication time (ST), sample mass of muscle tissues (SM), temperature of ultrasonic bath (T), nitric acid (A1), and mixture of acid and oxidant (A2) have been studied. From these studies, some variables showed significant effect on % recovery, and they were further optimized by a 23+star central composite design, which involved 16 experiments. Optimum values of the variables were selected for the development of USD to determine the contents of As, Cd, and Pb in muscle tissue of five fish species, used as bioindicators for Lake Manchar (Sindh, Pakistan) to know whether consumption of these fishes threatens human health. The determination of three toxic elements under study was carried out by electrothermal atomic absorption spectrometry (ETAAS). The accuracy of the optimized procedure was evaluated by analysis of certified reference materials DORM‐2 (Dogfish Muscle Certified Reference Material for Trace Elements) and by comparison with conventional wet acid digestion methodology. No significant differences were observed for p=0.05 when comparing the values obtained by the proposed USD method and conventional digestion method (CDM) (paired t‐test). The average relative standard deviation of the USD method varied between 4.05%, 7.53%, and 4.55% for As, Cd, and Pb, respectively (n=10).