Determination of Total Phenolic Content and Total Flavonoid Content of Nutmeg Flesh (Myristica Fragrans Houtt) Ethanol Extract from South Aceh Province

Abstract

The nutmeg plant (Myristica fragrans Houtt) is often used as a medicinal ingredient and has medicinal efficacy. Nutmeg samples were obtained from South Aceh district, which is the main commodity obtained in the area. This study aims to measure the total phenolic content (TPC) and total flavonoid content (TFC) of the ethanol extract of nutmeg flesh. The dried nutmeg flesh was extracted (maceration) using an ethanol solvent, and the extract weight was 18.35%. The TPC and TFC analysis use the UV-visible spectrophotometer method and are measured at wavelengths of 759 nm and 495 nm, respectively. Gallic acid standards were used to measure total phenolic levels and quercetin standards to measure total flavonoid levels. Data of linearity, R square (R2), LoD, and LoQ were also calculated for each standard. The TPC and TFC contents in the ethanol extract of nutmeg flesh were found to be 76.972 ± 0.97 mg GAE/g and 15.625 ± 0.26 mg QE/g respectively. The presence of phenolic compounds and flavonoids shows that the ethanol extract of nutmeg flesh has high value metabolites that show good biological activity.