Total Phenolic and Flavonoid Content and Antibacterial Properties ofPolygonatum orientaleDesf andTiliadasystyla

Abstract

Background: Secondary metabolites of plants such as phenol and flavonoids have an extreme potential for neutralizing free radicals. The antioxidant and cytotoxic activities of plants are related to phenolic or flavonoids compounds. The occurrence of drug resistance to antimicrobial drugs has led to use of medicinal herbs in treatment of infections. Antibiotic resistant of Staphylococcus aureus has become a major problem in the treatment of diseases. Objectives: The aim of this study was determination of total phenolic content (TPC) and total flavonoids content (TFC) of Polygonatum orientale Desf and Tilia dasystyla and evaluation of their anti-bacterial effects on Staphylococcus aureus bacterium. Investigation of TPC of P. orientale Desf and T. dasystyla has not been reported before. Methods: Total phenolic and flavonoid content of P. orientale Desf and T. dasystyla extracts were determined using colorimetric methods of Folin-Ciocalteu and aluminum chloride. Antimicrobial activities of the extracts were evaluated by microdilution broth and disc diffusion methods to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Results: The results showed that TFC of P. orientale Desf with the value of 7.9 ± 0.040 mg/g DW extracted with diluted water solvent and boiling method and TPCs of T. dasystyla with the value of 62.13 ± 0.073 mg/g DW extracted with methanol solvent and boiling method were the highest amount. Methanol extract of P. orientale Desf had more antibacterial activities with the MBC and MIC values of 0.140 mg/mL and 8±0.4 mm zone of inhibition. Conclusion: Tilia dasystyla and P. orientale Desf contain bioactive compounds such as phenolic and flavonoids that can be used as promising option in pharmacognostical studies for treatment of S. aureus infections.