﻿Determination of TLC fingerprint biomarker of Ipomoea batatas (L.) Lam leaves extracted with ethanol and its potential as antihyperglycemic agent

Putu Sanna Yustiantara,Ni Kadek Warditiani,Yan Ramona,Ni Luh Kade Arman Anita Dewi,I Made Jawi,Pande Made Nova Armita Sari,I Made Agus Gelgel Wirasuta

doi:10.3897/pharmacia.68.e71334

Abstract

Antihyperglycemic activity of Ipomoea batatas (L.) leaves has been studied for years. As many naturally found varieties of such plant species have been used as herbal medicines, there is a need to establish a fingerprint biomarker standardization method. Our research combined TLC fingerprints and chemometric analysis to determine biomarkers in leaf ethanol extract of Ipomoea batatas (L.) (EIBL). We predicted the main constituents of anthocyanin glycosides and their acylated derivates in the leaf ethanol extract of such plants using ESI-MS. The EIBL that contained identified biomarkers has succeeded in providing an antihyperglycemic effect in experimental using STZ-induced rats. This study also found a linear correlation between doses of flavonoid derivates as antioxidant agents with their antihyperglycemic activities. Therefore, this biomarker information can be used as a model to predict the dose-responses of the antihyperglycemic activities produced by other leaf ethanol extracts of I. batatas.

Highlights

Ipomoea batatas (L.) has been reported to have beneficial effect by lowering blood glucose (BG) in type 2 diabetic patients (Ludvik et al 2003, 2008, Zhao et al 2007, Ooi and Loke 2013, Daud et al 2016, Rafi U and Luka 2018)
This study demonstrated the use of TLC fingerprints to determine biomarker compounds in leaf ethanol extract of Ipomoea batatas (L.) from Bali (IBL) (EIBL)
Linear correlation of selected λmax of antioxidant biomarker was determined from multivariate analysis

Summary

Introduction

Ipomoea batatas (L.) has been reported to have beneficial effect by lowering blood glucose (BG) in type 2 diabetic patients (Ludvik et al 2003, 2008, Zhao et al 2007, Ooi and Loke 2013, Daud et al 2016, Rafi U and Luka 2018). Indonesia has around 50 Ipomoea batatas (L.) cultivars (Rahajeng 2015), and among those cultivars, Ipomoea batatas (L.) from Bali (IBL). The leaves of IBL are lobed with pointed tips, young and old leaf’s colors are dark purple, while the stems and leaf bones are purple. Its flower is a star-shaped flower crown, with the tip of the flower petals tapered, and the petals are pale purple. The tuber skin is purplish-white, with white flesh tuber.

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