Abstract
Chromatographic separation of methylxanthine metabolites was achieved using a Hypersil octadecylsilane column with a simple concave gradient elution programme of 0–12.75% acetonitrile in 1% tetrahydrofuran, pH 4.8, and the eluted components were detected by monitoring their absorption at 280 nm. An extraction procedure involving the formation of an ion-pair complex was developed which gave significant improvements over previously described methods including a shorter chromatographic run of 20 min. A thorough comparison of this procedure with a more convenient alternative involving direct injection of diluted urine specimens showed that the latter analysis was adequate for the quantitation of the major urinary metabolites of caffeine and theophylline.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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