Abstract
A gas chromatographic method for the determination of the nicotine metabolite trans-3′-hydroxycotinine is described. The method involves conversion of the metabolite to the tert.-butyldimethylsilyl derivative, chromatography on a fused-silica capillary column, and determination using nitrogen—phosphorus detection or electron ionization mass spectrometry with selected ion monitoring. A structural analogue, trans-3-hydroxy-1-methyl-5-(2-pyridyl)pyrrolidin-2-one ( trans-3′-hydroxy- ortho-cotinine), was used as an internal standard. Using selected ion monitoring, good precision and accuracy were obtained for determination of trans-3′-hydroxycotinine in urine over the concentration range 10–10 000 ng/ml. There was a good correlation between concentrations determined by selected ion monitoring and by nitrogen—phosphorus detection in urine of smokers, although low concentrations determined using nitrogen—phosphorus detection tended to be somewhat higher, suggesting some interference from urinary constituents. Concentrations and 24-h excretion of trans-3′-hydroxycotinine in the urine of 22 cigarette smokers are reported and compared to concentrations and excretion of nicotine, cotinine, nicotine 1′-N-oxide, nornicotine, and cotinine N-oxide.
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More From: Journal of Chromatography B: Biomedical Sciences and Applications
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