Determination of the Critical Micelle Concentration of Triton X-100 Using the Compound 3-(benzoxazol-2-yl)-7-(N,N-diethylamino)chromen-2-one as Fluorescent Probe

Abstract

In the present study we estimated the Critical Micelle Concentration (CMC) of Triton X-100 in an aqueous buffered medium (PBS buffer, pH 7.4), using 3-(benzoxazol-2-yl)-7-(N,N-diethylamino)chromen-2-one (BDC), a push-pull compound, as extrinsic fluorescence probe. The CMC value found, 0.33 mmol L -1 , shows good agreement with data from literature obtained using the fluorescence probe technique. Additionally, the polarity, in terms of the E T (30) scale, and the viscosity of the micelle microenvironment in which the probe is preferentially allocated, was estimated as being 46.9 kcal mol -1 and 70.3 cP, respectively, confirming that this microdomain is polar and highly viscous, with characteristics of polyethylene glycol groups consisting in the palisade layer at the micelle-water interface. DOI: http://dx.doi.org/10.17807/orbital.v0i0.923