Comment on ‘Critical micellar concentration and protein–surfactant interaction (Comment to ‘Destructive and protective action of sodium dodecyl sulphate micelles on the native conformation of Bovine Serum Albumin: A study by extrinsic fluorescence probe 1-hydroxy-2-naphthaldehyde’)’

Abstract

Commenting on the Letter entitled ‘Destructive and protective action of sodium dodecyl sulphate micelles on the native conformation of Bovine Serum Albumin: A study by extrinsic fluorescence probe 1-hydroxy-2-naphthaldehyde’ [Chem. Phys. Lett. 463 (2008) 183], this short contribution aims to clarify that the critical micellar concentration (CMC) of a charged surfactant strongly depends on the ionic strength. Main features of fluorimetric determinations of the CMC are also briefly discussed. In general, the study of surfactant-induced protein transitions will greatly benefit from independent ‘blank’ experiments to evaluate the CMC of the surfactant under the conditions of the protein assays.