Determination of the concentration of metoprolol tartrate in human plasma by HPLC coupled with hollow fiber liquid phase microextraction method

Abstract

Objective: To establish a HPLC coupled with hollow fiber liquid phase microextraction(HF-LPME) method for determination of the concentration of metoprolol tartrate in human plasma.Methods: The concentrations of donor phase and receptive phase,time,temperature and rotary speed of extraction,and concentration of NaCl in HF-LPME were optimized.The hollow fiber was placed in plasma sample solution to perform microextraction,and then the extract was analyzed by HPLC method with fluorescence detection on Agilent Zorbax XDB-C18 column.The mobile phase consisted of methanol-0.1% phosphoric acid(40∶60) with a flow rate of 1 ml/min.The excitation wavelength was 227 nm and the emission wavelength was 305 nm.The column temperature was 30 ℃.Results: Metoprolol tartrate was in good linearity within the range of 2-125 ng/ml.The intra-day and inter-day RSD of low,middle and high concentrations(5,20,100 ng/ml) were all less than 10%,and recoveries were(87.1±7.3)%,(92.6±5.8)% and(89.1±2.5)%,respectively.Conclusion:HPLC coupled with HF-LPME method is suitable for analysis of concentration of metoprolol tartrate in human plasma.