Abstract
BackgroundNowadays it remains a controversial issue whether a correlation exists between the apoptosis rate of tumor tissue and the prognosis of the patients. We aimed to explore the prognostic significance of apoptosis index of human osteosarcoma tissue.MethodsThe technique of terminal DNA breakpoints in situ 3 - hydroxy end labeling (TUNEL) was used to detect and analysis apoptosis index in 56 osteosarcoma specimens. The relationships between apoptosis index of tumor tissue and long term survival of patients as well as pathologic classification, tumor clinical stages, tumor size and level of serum alkaline phosphatase were analyzed.ResultsOur studies showed the cases with high apoptosis index had significantly longer survival time. Apoptosis index in osteosarcoma tissue was correlated with tumor size and level of serum alkaline phosphatase but not with pathologic classifications and clinical stages of tumor.ConclusionOur results demonstrated that apoptosis index of osteosarcoma tissue combined with serum alkaline phosphatase could used as valid indicators to predicate the malignant level and prognosis of osteosarcoma cases, which would contribute to enhance efficacy of clinical treatments for osteosarcoma.
Highlights
Osteosarcoma is the most frequent highly malignant bone-tumor with a peak manifestation during the second and third decade of life
Relationship between gross tumor volume and AI of osteosarcoma Our study showed that the grade of apoptotic index in osteosarcoma tissue of gross tumor volume ≥ 10cm was significantly different compared with that of gross tumor volume < 10 cm (Table 1)
Wilcoxon W rank test revealed the grade of AI in the alkaline phosphatase (ALP) elevated group was significantly different compared with that in the normal ALP group (Table 2)
Summary
Osteosarcoma is the most frequent highly malignant bone-tumor with a peak manifestation during the second and third decade of life. It’s the recent realization of malignant tumors may grow due to limitless proliferation and athanasy of. We hypothesized that it were the unstable experimental techniques for detecting different phase of apoptosis of tumor cells that had influenced the results of apoptotic rates. The relationships between apoptotic index of osteosarcoma tissue and the long-term survival of patients and interrelated influencing factors were analyzed. Nowadays it remains a controversial issue whether a correlation exists between the apoptosis rate of tumor tissue and the prognosis of the patients. We aimed to explore the prognostic significance of apoptosis index of human osteosarcoma tissue
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