Determination of teicoplanin in human plasma by reverse micelle mediated dispersive liquid-liquid microextraction with high performance liquid chromatography

Abstract

A reverse micelle mediated dispersive liquid-liquid microextraction (RM-DLLME) combined with high performance liquid chromatography-ultraviolet detector (HPLC-UV) was developed for extraction and determination of 5 A2 components of teicoplanin (TA2-1, TA2-2, TA2-3, TA2-4, TA2-5) in human plasma, and the mechanism of RM-DLLME was analysed and explored. In this method, 80 µL of the reverse micelle solution of cetylpyridinium chloride/n-hexanol (15 mmol/L) was used as the extraction solvent for the separation, extraction and enrichment of the teicoplanin in plasma sample. All factors affecting the extraction efficiencies of the target analytes, such as the amounts of acetonitrile and chloroform, the type and volume of reverse micelle solution, pH and volume of sample phase, dispersant, salt addition, extraction mode and time, centrifugation rate and time, were investigated and optimized. Under the optimum conditions, the 5 A2 components of teicoplanin achieved effective enrichment with the enrichment factors of 228-347 and obtained good linearity in the range of 0.8375-100.5 µg/mL with correlation coefficients higher than 0.9960. The limits of detection were ranged between 0.5025-3.015 µg/mL. Relative standard deviation values of the method precisions were lower than 10.6% and the average recoveries were in the range of 82.7-111.3%. The determination results of the method were demonstrated with favorable characteristics, such as high enrichment, good selectivity and sensitivity, satisfactory precision and accuracy, and this method could be employed to analysis of the teicoplanin in human plasma samples.