Determination of spontaneous dicentric frequencies and establishment of dose-response curves after expose of human peripheral blood lymphocytes to low- and high-dose rate 60Co gamma rays – the basis for cytogenetic biodosimetry in Vietnam

Abstract

Purpose: The purposes of this study are to investigate spontaneous dicentric frequencies and dose–response curves of dicentrics induced by gamma 60Co for replenishing the data sets used for biodosimetry in Vietnam.Materials and methods: One hundred and four healthy donor blood samples were collected for chromosome aberrations background study, 03 healthy donor blood samples were used for generating the dose–response curves at 1.96 mGy/min and 275 mGy/min. Blood collection, in vitro irradiation, cell culture and harvest, slide preparation and metaphase scoring were performed according to IAEA standard protocol (2011). Blind exposed samples were scored for verifying each curve.Results: The dicentric, fragment and chromatid break frequencies in 106,310 metaphases of 104 donors were 0.023% ± 0.005%, 0.045% ± 0.007% and 0.101% ± 0.011%, respectively. The dose–response curve for low-dose rate was y = C + (0.0137 ± 0.0055)D + (0.0912 ± 0.0142)D2 and for high-dose rate was y = C + (0.0337 ± 0.0046)D + (0.0539 ± 0.0031)D2, where both of them were verified.Conclusion: The data of this study were established for biological dose assessment in cases with low LET of accidental or occupational radiation exposures in the dose range of 0.1–5.0 Gy.