Determination of Some Human Enzymes Activities in Presence of Titanium Oxide Nanoparticles

Abstract

Titanium oxide nanoparticles (TiO2NPs) are the most common particles that have industrial, commercial, medial and antibacterial applications. On the other hand, they have toxic properties within some concentrations that show the ability to inhibit some human enzymes. In this study a diluted solutions of TiO2NPs were prepared by weighting(0.08 gm)with deionized water as(10−1, 10−2, 10−3, 10−4, 10−5) with low conductivity to avoid interaction with other ions. Our study aimed to estimate the effect of different concentration of TiO2NPs on liver enzymesaspartate transaminase (GOT), alanine transaminase (GPT), alkaline phosphatase(ALP) and Amylase in human serum by using a healthy human serum with a mixture of enzyme solution in absence and presence of diluted solution of TiO2NPs. The results showed that different TiO2NPs concentrations used had inhibitory effect on all the enzymes.