Abstract
Procaine, adiphenine, drofenine, nafronyl, tetracaine, and meclofenoxate and their degradation products were separated by reversed phase high performance liquid chromatography (HPLC) on Spherisorb ODS 2 columns (125 or 250 × 4 mm). The mobile phase was a mixture of acetonitrile or methanol and a buffer solution (pH=4.5) of sodium acetate (0.02 M) and acetic acid. Seven amines with different chemical structures were added respectively into the buffer solution in order to modify the peak tailing and retention behavior of the compounds analyzed. Significant improvement in peak shape and retention time of basic drugs was observed by adding some amines, while in the case of their degradated acids these properties were not modified. Triethylamine was chosen to analyze all the pharmaceuticals studied in this work except for drofenine where di-n-butylamine appeared the best amine.
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