Abstract
Methods are described for the determination of selenols (RSeH), diselenides (RSeSeR), and selenenyl sulfides (RSeSR'). The methods are based on the separation of the selenium-containing compounds by reversed-phase liquid chromatography followed by detection with an electrochemical detector with dual Hg/Au amalgam electrodes in series in the eluent stream. Selenols are detected directly by facilitation of the oxidation of Hg from the downstream electrode when it is set to +0.150 V vs the Ag/AgCl reference electrode. Diselenides and selenenyl sulfides are determined by first reducing them to the selenol and/or thiol form at the upstream electrode followed by detection of the selenol and/or thiol at the downstream electrode. By appropriate choice of potential for the upstream electrode, diselenides, and selenenyl sulfides can be determined selectively in mixtures containing diselenides, selenenyl sulfides, and disulfides. The results indicate that high-performance liquid chromatography with electrochemical detection is a sensitive and selective method for the determination of selected selenium-containing compounds.
Published Version
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