Determination of Secondary Nanostructure of Model Membrane from Local Fishes for Application in Pharmaceutical Field

Abstract

As it is di cult to obtain human stratum corneum for testing the pharmacological e ect of the drugs, many researchers have tried to nd alternative membrane for this purpose, instead of the human skin. Many animal skins e.g. pig, reptile, or sh skin seem to be suitable for this purpose. Therefore this project will provide more details to help understand more deeply the structure of these alternative skins. This project is focused on the sh skin because such skin can be easily found in Germany. Two di erent techniques i.e. small and wide angle X-ray scattering (SWAXS) and di erential scanning calorimetry (DSC) have been applied for characterization of some local sh skins. The results showed that di erent skins (i.e. human stratum corneum, pig, shed snake, and sh) showed small di erences in SWAXS patterns as well as in DSC thermograms. The drug paracetamol and the surfactant sodium lauryl sulphate can penetrate into the sh skins and they can a ect a rearrangement of the inner structure which contains di erent lipids and proteins. The data are in agreement with those obtained for the shed snake skins in our previous study.